ExperimentalBiochemistry
Tyrosinase Characterization
Protein Purification and Enzymatic Profiling
Isolated and purified tyrosinase by chromatographic separation, then verified purity and enzyme activity through SDS-PAGE and kinetic assays.
Overview
Executed a complete purification workflow including affinity and size-exclusion chromatography, verified purity by SDS-PAGE, and quantified enzymatic activity under varying substrate and inhibitor conditions to evaluate functional behavior.